产品简介：

       结晶紫（Crystal Violet）是最初在革兰氏染色技术中用于区分革兰氏阴性和革兰氏阳性菌的染料之一，染色原理在于不同的细菌其细胞壁化学结构不同。革兰氏阳性菌，即染色呈阳性菌体内保留有结晶紫-碘复合物，在显微镜下呈紫褐色。在组织培养试验中结晶紫可用于对细菌污染情况的评估。

   Introduction

       Crystal Violet Stain Solution, 2.5% is mainly composed of ammonium oxalate, crystal violet, ethanol etc. The solution is a component of Gram stain. The primary stain is crystal violet, a basic dye taken up by all bacteria due to its ability to rapidly permeate the cell wall. It stains the protoplast of bacteria purple. The potassium-iodine mixture is the mordant which complexes with the primary stain in the cell. In gram-positive cells, the crystal violet-iodine complex is trapped in the cell due to a decrease in cell wall permeability caused by alcohol dehydration. Gram-positive cells are stained purple. In gram-negative cells, the complex is removed by the decolorizer due to an increase in permeability caused by solubility of the lipids in alcohol. The counterstain used must be a contrasting color to the primary stain and, in this case, it is safranin which stains gram-negative cells red.